Details of the Target

General Information of Target

Target ID LDTP04724
Target Name GDNF family receptor alpha-1 (GFRA1)
Gene Name GFRA1
Gene ID 2674
Synonyms
GDNFRA; RETL1; TRNR1; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFLATLYFALPLLDLLLSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNF
SLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSP
YEPVNSRLSDIFRVVPFISDVFQQVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTT
SVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREK
PNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPN
YIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPV
QTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNY
EKEGLGASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS
Target Bioclass
Other
Family
GDNFR family
Subcellular location
Cell membrane
Function Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
Uniprot ID
P56159
Ensemble ID
ENST00000355422.11
HGNC ID
HGNC:4243
ChEMBL ID
CHEMBL3833481

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCLP1 SNV: p.L425F .
EFO27 Deletion: p.F264LfsTer15 .
GB1 SNV: p.F328L .
HKGZCC Deletion: p.P11RfsTer11 .
HT115 SNV: p.F328L .
JHH1 SNV: p.C277Y .
NCIH2286 SNV: p.D350Y .
NCIH716 SNV: p.P356A .
RKO SNV: p.R88C .
SKMEL3 SNV: p.N86T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C36(3.95); C52(5.99)  LDD3345  [1]
BTD
 Probe Info  		C52(1.19)  LDD2141  [2]
IA-alkyne
 Probe Info  		C267(0.00); C277(0.00); C167(0.00)  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_5
 Probe Info  		C167(0.00); C161(0.00); C42(0.00); C221(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C395(20.00); C36(0.67)  LDD2227  [4]
 LDCM0372  CL103 HEK-293T C216(0.62)  LDD1576  [5]
 LDCM0376  CL107 HEK-293T C216(0.76)  LDD1580  [5]
 LDCM0381  CL111 HEK-293T C216(0.98)  LDD1585  [5]
 LDCM0385  CL115 HEK-293T C216(0.62)  LDD1589  [5]
 LDCM0389  CL119 HEK-293T C216(0.82)  LDD1593  [5]
 LDCM0394  CL123 HEK-293T C216(0.76)  LDD1598  [5]
 LDCM0398  CL127 HEK-293T C216(0.69)  LDD1602  [5]
 LDCM0402  CL15 HEK-293T C216(0.67)  LDD1606  [5]
 LDCM0415  CL27 HEK-293T C216(0.89)  LDD1619  [5]
 LDCM0418  CL3 HEK-293T C216(0.89)  LDD1622  [5]
 LDCM0428  CL39 HEK-293T C216(0.94)  LDD1632  [5]
 LDCM0455  CL63 HEK-293T C216(0.67)  LDD1658  [5]
 LDCM0481  CL87 HEK-293T C216(1.52)  LDD1684  [5]
 LDCM0494  CL99 HEK-293T C216(0.88)  LDD1697  [5]
 LDCM0634  CY-0357 Hep-G2 C36(1.37); C233(0.69); C395(0.25)  LDD2228  [4]
 LDCM0495  E2913 HEK-293T C216(0.64)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C216(1.20)  LDD1671  [5]
 LDCM0022  KB02 786-O C87(3.25)  LDD2247  [1]
 LDCM0023  KB03 769-P C42(1.28)  LDD2663  [1]
 LDCM0024  KB05 NCI-H1650 C36(3.95); C52(5.99)  LDD3345  [1]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C52(1.19)  LDD2141  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sortilin-related receptor (SORL1) VPS10-related sortilin family Q92673

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402