Details of the Target

General Information of Target

Target ID LDTP04654
Target Name Solute carrier family 12 member 3 (SLC12A3)
Gene Name SLC12A3
Gene ID 6559
Synonyms
NCC; TSC; Solute carrier family 12 member 3; Na-Cl cotransporter; NCC; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNT
IDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEG
EAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVT
VTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAE
TVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANY
LVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANI
SGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLA
CSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVF
QCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSY
ALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLL
LYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVD
FVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRG
VQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGL
NVSKMMQAHINPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDG
GLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDIN
QNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLN
EIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYC
Q
Target Type
Successful
Target Bioclass
Transporter and channel
Family
SLC12A transporter family
Subcellular location
Cell membrane
Function
Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1.
TTD ID
T84886
Uniprot ID
P55017
DrugMap ID
TTP362L
Ensemble ID
ENST00000438926.6
HGNC ID
HGNC:10912
ChEMBL ID
CHEMBL1876

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAKI1 SNV: p.A749V .
DEL SNV: p.L1001F .
HCC1419 SNV: p.T553S .
Ishikawa (Heraklio) 02 ER SNV: p.P651S .
JURKAT SNV: p.F427I .
KASUMI2 SNV: p.D29G .
LS180 SNV: p.E609K .
NCIH2172 SNV: p.V404F .
NCIH3122 SNV: p.G116W .
NCIH358 Substitution: p.R158L .
RKO SNV: p.G980W .
SW1783 SNV: p.V807E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TFBX
 Probe Info  		C16(0.00); C1020(0.00)  LDD0148  [1]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 9 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Bendroflumethiazide Small molecular drug D02PAH
Benzthiazide Small molecular drug DB00562
Chlorothiazide Small molecular drug DB00880
Hydrochlorothiazide Small molecular drug DB00999
Indapamide Small molecular drug DB00808
Metolazone Small molecular drug D01WLC
Polythiazide Small molecular drug D06TNG
Quinethazone Small molecular drug DB01325
Trichlormethiazide Small molecular drug DB01021

References

1 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255