Details of the Target

General Information of Target

Target ID LDTP04361
Target Name Serpin B8 (SERPINB8)
Gene Name SERPINB8
Gene ID 5271
Synonyms
PI8; Serpin B8; Cytoplasmic antiproteinase 2; CAP-2; CAP2; Peptidase inhibitor 8; PI-8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDDLCEANGTFAISLFKILGEEDNSRNVFFSPMSISSALAMVFMGAKGSTAAQMSQALCL
YKDGDIHRGFQSLLSEVNRTGTQYLLRTANRLFGEKTCDFLPDFKEYCQKFYQAELEELS
FAEDTEECRKHINDWVAEKTEGKISEVLDAGTVDPLTKLVLVNAIYFKGKWNEQFDRKYT
RGMLFKTNEEKKTVQMMFKEAKFKMGYADEVHTQVLELPYVEEELSMVILLPDDNTDLAV
VEKALTYEKFKAWTNSEKLTKSKVQVFLPRLKLEESYDLEPFLRRLGMIDAFDEAKADFS
GMSTEKNVPLSKVAHKCFVEVNEEGTEAAAATAVVRNSRCSRMEPRFCADHPFLFFIRHH
KTNCILFCGRFSSP
Target Bioclass
Other
Family
Serpin family, Ov-serpin subfamily
Subcellular location
Cytoplasm
Function Has an important role in epithelial desmosome-mediated cell-cell adhesion.
Uniprot ID
P50452
Ensemble ID
ENST00000353706.6
HGNC ID
HGNC:8952

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.A330P DBIA    Probe Info 
EFO27 Deletion: p.E21KfsTer13 .
MDST8 SNV: p.P232S .
MEWO SNV: p.P32L DBIA    Probe Info 
NCIH716 SNV: p.A330P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K263(10.00)  LDD0277  [1]
m-APA
 Probe Info  		13.57  LDD0403  [2]
DBIA
 Probe Info  		C98(2.32)  LDD3310  [3]
BTD
 Probe Info  		C108(0.41)  LDD2096  [4]
IPM
 Probe Info  		C364(6.51)  LDD1701  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C348(0.00); C59(0.00); C98(0.00); C364(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C348(0.00); C59(0.00); C98(0.00); C364(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C348(0.00); C98(0.00); C364(0.00); C368(0.00)  LDD0037  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
Compound 10
 Probe Info  		N.A.  LDD2216  [7]
Compound 11
 Probe Info  		N.A.  LDD2213  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [6]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 13.57  LDD0403  [2]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C364(1.93)  LDD1702  [4]
 LDCM0022  KB02 T cell-activated C364(17.77)  LDD1708  [9]
 LDCM0023  KB03 MDA-MB-231 C364(6.51)  LDD1701  [4]
 LDCM0024  KB05 COLO792 C98(2.32)  LDD3310  [3]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C108(0.41)  LDD2096  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C108(1.72)  LDD2122  [4]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.