Details of the Target

General Information of Target

Target ID LDTP04035
Target Name DNA repair protein RAD52 homolog (RAD52)
Gene Name RAD52
Gene ID 5893
Synonyms
DNA repair protein RAD52 homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGG
QKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSY
HEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPR
QLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCS
SRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHST
PVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLAL
NNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS
Target Bioclass
Other
Family
RAD52 family
Subcellular location
Nucleus
Function
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Uniprot ID
P43351
Ensemble ID
ENST00000358495.8
HGNC ID
HGNC:9824
ChEMBL ID
CHEMBL2362978

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CASKI SNV: p.E122K .
COLO678 SNV: p.S53I .
HKGZCC SNV: p.R396H .
LS123 SNV: p.R227S .
NCIH716 SNV: p.S53I; p.R227S .
SNU1 SNV: p.W386C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C64(3.02)  LDD3310  [1]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A2058 C64(1.83)  LDD2253  [1]
 LDCM0023  KB03 A2058 C64(2.42)  LDD2670  [1]
 LDCM0024  KB05 COLO792 C64(3.02)  LDD3310  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
mRNA-decapping enzyme 1A (DCP1A) DCP1 family Q9NPI6
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (WRN) Helicase family Q14191
Serine/threonine-protein kinase PLK3 (PLK3) Ser/Thr protein kinase family Q9H4B4
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Importin subunit alpha-4 (KPNA3) Importin alpha family O00505
Importin subunit alpha-6 (KPNA5) Importin alpha family O15131
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein MOX-1 (MEOX1) . P50221
Paired box protein Pax-5 (PAX5) . Q02548
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA repair protein RAD52 homolog (RAD52) RAD52 family P43351
DNA repair protein RAD51 homolog 1 (RAD51) RecA family Q06609
Replication protein A 14 kDa subunit (RPA3) Replication factor A protein 3 family P35244
RAD52 motif-containing protein 1 (RDM1) . Q8NG50

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004