Details of the Target

General Information of Target

Target ID LDTP03532
Target Name M-phase inducer phosphatase 2 (CDC25B)
Gene Name CDC25B
Gene ID 994
Synonyms
CDC25HU2; M-phase inducer phosphatase 2; EC 3.1.3.48; Dual specificity phosphatase Cdc25B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMH
DLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMD
PHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEA
GSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPD
RKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKT
LEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQ
QEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETM
VALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKR
VILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQ
DYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
MPI phosphatase family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. The three isoforms seem to have a different level of activity.
TTD ID
T46365
Uniprot ID
P30305
DrugMap ID
TTR0SWN
Ensemble ID
ENST00000245960.10
HGNC ID
HGNC:1726
ChEMBL ID
CHEMBL4804

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.R543P .
CAOV3 SNV: p.A124S .
HT115 SNV: p.Y396Ter .
MDAMB157 SNV: p.I132V .
MOLT4 SNV: p.W217S; p.V432L .
NUGC3 SNV: p.F256L .
P31FUJ Deletion: p.S83_R84del .
SKMM2 SNV: p.T54N .
SKOV3 SNV: p.R93Q .
SNU1 SNV: p.P331S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C475(0.75)  LDD1701  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
WYneN
 Probe Info  		N.A.  LDD0021  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C475(0.63)  LDD2187  [5]
 LDCM0586  Fragment28 Ramos C475(0.94)  LDD2198  [5]
 LDCM0023  KB03 MDA-MB-231 C475(0.75)  LDD1701  [1]
 LDCM0024  KB05 Ramos C475(0.42)  LDD2185  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein beta/alpha (YWHAB) 14-3-3 family P31946
14-3-3 protein eta (YWHAH) 14-3-3 family Q04917
Proteasome activator complex subunit 3 (PSME3) PA28 family P61289

The Drug(s) Related To This Target

Phase 3
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Avastin+/-tarceva Small molecular drug D0C0NH
Investigative
Click To Hide/Show 16 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
2-sulfhydryl-ethanol Small molecular drug D0Q5FV
3-isopropyl-4-(Phenylamino)Naphthalene-1,2-dione Small molecular drug D0X5WD
3-isopropyl-4-(Phenylthio)Naphthalene-1,2-dione Small molecular drug D08GWQ
3-isopropyl-4-phenylnaphthalene-1,2-dione Small molecular drug D07JRA
4-(P-toluidino)-3-isopropylnaphthalene-1,2-dione Small molecular drug D0N7YT
4-ethoxynaphthalene-1,2-dione Small molecular drug D07REK
5,6,7,8-tetrahydroanthracene-1,4-dione Small molecular drug D0SI7S
6,7-dibromoquinoline-5,8-dione Small molecular drug D02UHP
Adociaquinone B Small molecular drug D07FFL
Anthraquinone Small molecular drug D0L5LN
Cysteine Sulfenic Acid Small molecular drug D0V6UC
Cysteinesulfonic Acid Small molecular drug D05AMX
Double Oxidized Cysteine Small molecular drug D0H1GU
L-cysteic Acid Small molecular drug DB03661
Methyl Mercury Ion Small molecular drug D09CZV
Nsc-95397 Small molecular drug D0J1KH
Discontinued
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Mx-7065 . D06REJ

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
5 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578