Details of the Target

General Information of Target

Target ID LDTP03492
Target Name Nitric oxide synthase 1 (NOS1)
Gene Name NOS1
Gene ID 4842
Synonyms
Nitric oxide synthase 1; EC 1.14.13.39; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Nitric oxide synthase, brain; bNOS; Peptidyl-cysteine S-nitrosylase NOS1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQA
GDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTI
RVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAP
RPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQV
DRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPS
KCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLF
PLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRC
VGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVW
NSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQ
IPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGV
RDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSAT
ESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHV
WKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCE
IFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQ
EERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAF
GHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANN
SLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFV
RLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGV
ISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEY
EEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIV
SYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAP
FRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSRE
PDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAE
DAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
NOS family
Subcellular location
Cell membrane, sarcolemma
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
TTD ID
T16117
Uniprot ID
P29475
DrugMap ID
TTZUFI5
Ensemble ID
ENST00000317775.11
HGNC ID
HGNC:7872
ChEMBL ID
CHEMBL3568

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL120 SNV: p.K1376R .
CCK81 SNV: p.P526S .
COLO678 SNV: p.G297A .
COLO800 SNV: p.R1369C .
DU145 SNV: p.K452N; p.P728T .
HCC15 SNV: p.G676C .
HCT15 SNV: p.D264Y .
HEC1 SNV: p.D602N .
HEC1B SNV: p.T761A .
HG3 SNV: p.G7A .
HL60 SNV: p.V316A .
HSC3 SNV: p.N277H .
HT115 SNV: p.S17Y .
IPC298 Substitution: p.P878L .
JURKAT SNV: p.T438A .
KARPAS299 SNV: p.E1054Q .
KATOIII SNV: p.A893E .
MCC13 SNV: p.V93E .
MDAMB453 SNV: p.E1087K .
MFE319 SNV: p.R1203C .
NCIH2286 SNV: p.P180R .
OPM2 SNV: p.S379F .
P31FUJ SNV: p.Q1333R .
RKO SNV: p.T439M .
SKMEL28 SNV: p.S482F .
TC71 SNV: p.W716L DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C913(1.16)  LDD3344  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A673 C828(5.56); C895(2.63); C988(2.98); C913(2.32)  LDD2261  [1]
 LDCM0023  KB03 A673 C828(2.90); C895(4.04); C988(4.35); C913(3.72)  LDD2678  [1]
 LDCM0024  KB05 NCI-H146 C913(1.16)  LDD3344  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840