Details of the Target

General Information of Target

Target ID LDTP03089
Target Name Collagen alpha-1(V) chain (COL5A1)
Gene Name COL5A1
Gene ID 1289
Synonyms
Collagen alpha-1(V) chain
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTG
FCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIY
NEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLI
LDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHY
SPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAA
KETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEG
EENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPY
YDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLP
GPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVS
AQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPP
GPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDD
GERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPP
GQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPP
GPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGED
GPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGR
GTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFP
GPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPP
GPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPP
GPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPV
GLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEP
GPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPS
GAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAA
GPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPT
GEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLR
GIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEK
GDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHP
GPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKL
EIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTC
VFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQ
NVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVL
EIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMG
Target Bioclass
Other
Family
Fibrillar collagen family
Subcellular location
Secreted, extracellular space, extracellular matrix
Function
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Uniprot ID
P20908
Ensemble ID
ENST00000371817.8
HGNC ID
HGNC:2209
ChEMBL ID
CHEMBL2364188

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.S1735G
Substitution: p.L1734G		 .
COLO792 SNV: p.G958E; p.P1431L .
HCC44 SNV: p.V137F; p.K825N; p.P836A DBIA    Probe Info 
HCT116 Insertion: p.P1304TfsTer29 .
HCT15 SNV: p.P692H; p.H977Q .
HEC1 SNV: p.G60C; p.G453D; p.P1577Q .
HEC1B SNV: p.G453D; p.P1577Q .
HS936T SNV: p.G268S .
HT115 SNV: p.A85T .
ICC137 SNV: p.G1348A .
IGROV1 Deletion: p.G1024VfsTer50
SNV: p.V45A		 .
IPC298 Substitution: p.G1411K .
JURKAT SNV: p.G1661R .
KATOIII SNV: p.P1418L .
KU1919 SNV: p.G527R .
KYSE410 SNV: p.P1385L .
LNCaP clone FGC SNV: p.G943D .
MCC13 SNV: p.P857L DBIA    Probe Info 
MCC26 SNV: p.M198I DBIA    Probe Info 
MEWO SNV: p.E584K; p.P945S .
MFE280 SNV: p.G919A .
NCIH1993 SNV: p.G910V .
NCIH2172 SNV: p.D142Y; p.G664V DBIA    Probe Info 
NCIH2291 SNV: p.P851A .
NCIH358 SNV: p.Q1026H; p.P1256R; p.L1734V .
OVK18 Substitution: p.G1613T .
RL952 SNV: p.G607R .
SKMEL2 SNV: p.G1177S .
SKMEL5 Substitution: p.P996F .
SNGM SNV: p.G1051V .
SUIT2 SNV: p.S884C .
SW620 SNV: p.L1734V .
TE1 SNV: p.E289K .
UACC257 SNV: p.P741L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K57(1.11); K1708(1.70); K69(2.04); K605(2.05)  LDD3494  [1]
BTD
 Probe Info  		C62(2.01)  LDD2136  [2]
DBIA
 Probe Info  		C1645(0.92); C1662(0.92)  LDD0078  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0020  ARS-1620 HCC44 C1645(0.92); C1662(0.92)  LDD0078  [3]
 LDCM0022  KB02 A-172 C62(1.16)  LDD2251  [4]
 LDCM0023  KB03 A-172 C62(2.57)  LDD2668  [4]
 LDCM0024  KB05 NCI-H2172 C62(4.95)  LDD3356  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C62(2.01)  LDD2136  [2]

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840