Details of the Target

General Information of Target

Target ID LDTP03041
Target Name POU domain, class 3, transcription factor 2 (POU3F2)
Gene Name POU3F2
Gene ID 5454
Synonyms
BRN2; OCT7; OTF7; POU domain, class 3, transcription factor 2; Brain-specific homeobox/POU domain protein 2; Brain-2; Brn-2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Octamer-binding protein 7; Oct-7; Octamer-binding transcription factor 7; OTF-7
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATAASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLVQGDYGALQSNGHPLSHAHQ
WITALSHGGGGGGGGGGGGGGGGGGGGGDGSPWSTSPLGQPDIKPSVVVQQGGRGDELHG
PGALQQQHQQQQQQQQQQQQQQQQQQQQQRPPHLVHHAANHHPGPGAWRSAAAAAHLPPS
MGASNGGLLYSQPSFTVNGMLGAGGQPAGLHHHGLRDAHDEPHHADHHPHPHSHPHQQPP
PPPPPQGPPGHPGAHHDPHSDEDTPTSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYG
NVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTSIDKIAAQGRKRKKRTS
IEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGGTLP
GAEDVYGGSRDTPPHHGVQTPVQ
Target Bioclass
Transcription factor
Family
POU transcription factor family, Class-3 subfamily
Subcellular location
Nucleus
Function
Transcription factor that plays a key role in neuronal differentiation. Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides. Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes.
Uniprot ID
P20265
Ensemble ID
ENST00000328345.8
HGNC ID
HGNC:9215
ChEMBL ID
CHEMBL4189

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 Insertion: p.G81RfsTer80 .
HCT15 SNV: p.T13A; p.K330E .
HT115 SNV: p.I308V .
HUH7 SNV: p.R169L; p.A174P .
KATOIII SNV: p.P207L .
NCIH1703 SNV: p.G247C .
NUGC3 SNV: p.P258L .
OZ SNV: p.G73A .
RKO SNV: p.P152L .
RL952 SNV: p.A388V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C361(1.53)  LDD3413  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 IPC-298 C361(2.10)  LDD2400  [1]
 LDCM0023  KB03 IPC-298 C361(1.81)  LDD2817  [1]
 LDCM0024  KB05 RVH-421 C361(1.53)  LDD3413  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone acetyltransferase p300 (EP300) . Q09472
Transcription factor
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Paired box protein Pax-3 (PAX3) Paired homeobox family P23760
POU domain, class 3, transcription factor 2 (POU3F2) POU transcription factor family P20265
TATA-box-binding protein (TBP) TBP family P20226
Transcription factor SOX-10 (SOX10) . P56693

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853