Details of the Target

General Information of Target

Target ID LDTP02814
Target Name Transcription factor E2-alpha (TCF3)
Gene Name TCF3
Gene ID 6929
Synonyms
BHLHB21; E2A; ITF1; Transcription factor E2-alpha; Class B basic helix-loop-helix protein 21; bHLHb21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3; TCF-3; Transcription factor ITF-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNQPQRMAPVGTDKELSDLLDFSMMFPLPVTNGKGRPASLAGAQFGGSGLEDRPSSGSWG
SGDQSSSSFDPSRTFSEGTHFTESHSSLSSSTFLGPGLGGKSGERGAYASFGRDAGVGGL
TQAGFLSGELALNSPGPLSPSGMKGTSQYYPSYSGSSRRRAADGSLDTQPKKVRKVPPGL
PSSVYPPSSGEDYGRDATAYPSAKTPSSTYPAPFYVADGSLHPSAELWSPPGQAGFGPML
GGGSSPLPLPPGSGPVGSSGSSSTFGGLHQHERMGYQLHGAEVNGGLPSASSFSSAPGAT
YGGVSSHTPPVSGADSLLGSRGTTAGSSGDALGKALASIYSPDHSSNNFSSSPSTPVGSP
QGLAGTSQWPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLP
GHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPD
SYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTSPDEDEDDLLPP
EQKAEREKERRVANNARERLRVRDINEAFKELGRMCQLHLNSEKPQTKLLILHQAVSVIL
NLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQMVLSAPHPGLSEAHNPAGHM
Target Type
Literature-reported
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.; [Isoform E47]: Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.
TTD ID
T95715
Uniprot ID
P15923
DrugMap ID
TTULOD8
Ensemble ID
ENST00000262965.12
HGNC ID
HGNC:11633

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.A374D .
HCT15 SNV: p.E583K .
KMS11 SNV: p.A377V .
LS180 Deletion: p.P540QfsTer61 .
MFE319 Insertion: p.Q361PfsTer32 .
MOLT4 SNV: p.V598I .
NCIH1155 SNV: p.Q148Ter .
REC1 SNV: p.E501D .
SNGM SNV: p.L126M .
SNU1 Deletion: p.Q361RfsTer33 .
TE8 SNV: p.D395N .
TOV21G SNV: p.G99Ter; p.D383Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
NAIA_5
 Probe Info  		C576(20.00)  LDD2227  [1]
DBIA
 Probe Info  		C576(1.07)  LDD1571  [2]
AOyne
 Probe Info  		15.00  LDD0443  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C576(20.00)  LDD2227  [1]
 LDCM0367  CL1 HEK-293T C576(1.07)  LDD1571  [2]
 LDCM0370  CL101 HEK-293T C576(0.78)  LDD1574  [2]
 LDCM0374  CL105 HEK-293T C576(0.98)  LDD1578  [2]
 LDCM0378  CL109 HEK-293T C576(0.78)  LDD1582  [2]
 LDCM0383  CL113 HEK-293T C576(0.87)  LDD1587  [2]
 LDCM0387  CL117 HEK-293T C576(1.13)  LDD1591  [2]
 LDCM0392  CL121 HEK-293T C576(0.69)  LDD1596  [2]
 LDCM0396  CL125 HEK-293T C576(0.64)  LDD1600  [2]
 LDCM0400  CL13 HEK-293T C576(0.65)  LDD1604  [2]
 LDCM0413  CL25 HEK-293T C576(0.86)  LDD1617  [2]
 LDCM0426  CL37 HEK-293T C576(0.88)  LDD1630  [2]
 LDCM0439  CL49 HEK-293T C576(0.84)  LDD1643  [2]
 LDCM0453  CL61 HEK-293T C576(0.71)  LDD1656  [2]
 LDCM0466  CL73 HEK-293T C576(0.67)  LDD1669  [2]
 LDCM0479  CL85 HEK-293T C576(0.80)  LDD1682  [2]
 LDCM0492  CL97 HEK-293T C576(0.70)  LDD1695  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sodium/potassium-transporting ATPase subunit alpha-3 (ATP1A3) Cation transport ATPase (P-type) (TC 3.A.3) family P13637
Phospholipase D1 (PLD1) Phospholipase D family Q13393
26S proteasome non-ATPase regulatory subunit 9 (PSMD9) Proteasome subunit p27 family O00233
Ras-related protein Rab-5A (RAB5A) Rab family P20339
Transcription factor
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Achaete-scute homolog 1 (ASCL1) . P50553
Achaete-scute homolog 3 (ASCL3) . Q9NQ33
DNA-binding protein inhibitor ID-1 (ID1) . P41134
DNA-binding protein inhibitor ID-2 (ID2) . Q02363
DNA-binding protein inhibitor ID-3 (ID3) . Q02535
Forkhead box protein H1 (FOXH1) . O75593
Heart- and neural crest derivatives-expressed protein 1 (HAND1) . O96004
Myoblast determination protein 1 (MYOD1) . P15172
T-cell acute lymphocytic leukemia protein 1 (TAL1) . P17542
Twist-related protein 1 (TWIST1) . Q15672
Twist-related protein 2 (TWIST2) . Q8WVJ9
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Platelet endothelial cell adhesion molecule (PECAM1) . P16284
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-D1-binding protein 1 (CCNDBP1) CCNDBP1 family O95273
Vimentin (VIM) Intermediate filament family P08670
TRPM8 channel-associated factor 1 (TCAF1) TCAF family Q9Y4C2

References

1 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.