Details of the Target

General Information of Target

Target ID LDTP02623
Target Name Granzyme A (GZMA)
Gene Name GZMA
Gene ID 3001
Synonyms
CTLA3; HFSP; Granzyme A; EC 3.4.21.78; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRNSYRFLASSLSVVVSLLLIPEDVCEKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIA
KDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQL
MEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCN
DRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGP
GVYILLSKKHLNWIIMTIKGAV
Target Bioclass
Enzyme
Family
Peptidase S1 family, Granzyme subfamily
Subcellular location
Secreted
Function
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
Uniprot ID
P12544
Ensemble ID
ENST00000274306.7
HGNC ID
HGNC:4708
ChEMBL ID
CHEMBL4307

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.L8M .
MEWO SNV: p.G32E .
NCIH1703 SNV: p.T156I .
NCIH358 SNV: p.T52I .
SKMEL28 SNV: p.G32E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K98(5.00)  LDD0277  [1]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270