Details of the Target

General Information of Target

Target ID LDTP02039
Target Name Argininosuccinate lyase (ASL)
Gene Name ASL
Gene ID 435
Synonyms
Argininosuccinate lyase; ASAL; EC 4.3.2.1; Arginosuccinase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEM
DQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTD
LRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVAL
TRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVA
EFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKA
GRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENM
GQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLF
SGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA
Target Bioclass
Enzyme
Family
Lyase 1 family, Argininosuccinate lyase subfamily
Function
Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway.
Uniprot ID
P04424
Ensemble ID
ENST00000304874.14
HGNC ID
HGNC:746

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC1937 SNV: p.T181S .
HCT15 SNV: p.L136I .
JURKAT SNV: p.M60V .
SUDHL6 SNV: p.E4K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		12.41  LDD0402  [1]
STPyne
 Probe Info  		K97(5.76)  LDD0277  [2]
DBIA
 Probe Info  		C307(2.12)  LDD3352  [3]
IPM
 Probe Info  		C307(11.11)  LDD1701  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C264(0.00); C129(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
WYneN
 Probe Info  		N.A.  LDD0021  [6]
WYneO
 Probe Info  		N.A.  LDD0022  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
NAIA_5
 Probe Info  		C307(0.00); C264(0.00); C129(0.00)  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.80  LDD0403  [1]
 LDCM0367  CL1 HEK-293T C129(0.92)  LDD1571  [9]
 LDCM0370  CL101 HEK-293T C129(0.97)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C307(0.94)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C426(1.07)  LDD1576  [9]
 LDCM0374  CL105 HEK-293T C129(1.01)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C307(0.96)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C426(0.95)  LDD1580  [9]
 LDCM0378  CL109 HEK-293T C129(1.08)  LDD1582  [9]
 LDCM0380  CL110 HEK-293T C307(1.06)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C426(0.82)  LDD1585  [9]
 LDCM0383  CL113 HEK-293T C129(1.00)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C307(1.13)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C426(1.28)  LDD1589  [9]
 LDCM0387  CL117 HEK-293T C129(1.26)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C307(1.10)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C426(0.83)  LDD1593  [9]
 LDCM0392  CL121 HEK-293T C129(0.95)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C307(0.91)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C426(1.09)  LDD1598  [9]
 LDCM0396  CL125 HEK-293T C129(1.02)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C307(1.05)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C426(0.86)  LDD1602  [9]
 LDCM0400  CL13 HEK-293T C129(0.90)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C307(0.85)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C426(1.15)  LDD1606  [9]
 LDCM0407  CL2 HEK-293T C307(0.92)  LDD1611  [9]
 LDCM0413  CL25 HEK-293T C129(0.90)  LDD1617  [9]
 LDCM0414  CL26 HEK-293T C307(0.80)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C426(1.00)  LDD1619  [9]
 LDCM0418  CL3 HEK-293T C426(0.87)  LDD1622  [9]
 LDCM0426  CL37 HEK-293T C129(1.14)  LDD1630  [9]
 LDCM0428  CL39 HEK-293T C426(1.04)  LDD1632  [9]
 LDCM0439  CL49 HEK-293T C129(1.12)  LDD1643  [9]
 LDCM0441  CL50 HEK-293T C307(0.84)  LDD1645  [9]
 LDCM0453  CL61 HEK-293T C129(1.18)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C307(1.02)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C426(1.06)  LDD1658  [9]
 LDCM0466  CL73 HEK-293T C129(0.87)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C307(0.83)  LDD1670  [9]
 LDCM0479  CL85 HEK-293T C129(1.13)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C307(0.91)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C426(1.06)  LDD1684  [9]
 LDCM0492  CL97 HEK-293T C129(1.05)  LDD1695  [9]
 LDCM0493  CL98 HEK-293T C307(0.86)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C426(0.75)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C426(0.97)  LDD1698  [9]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C307(5.05)  LDD1702  [4]
 LDCM0468  Fragment33 HEK-293T C426(0.80)  LDD1671  [9]
 LDCM0427  Fragment51 HEK-293T C307(1.01)  LDD1631  [9]
 LDCM0022  KB02 769-P C307(1.49)  LDD2246  [3]
 LDCM0023  KB03 MDA-MB-231 C307(11.11)  LDD1701  [4]
 LDCM0024  KB05 NCI-H1975 C307(2.12)  LDD3352  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Argininosuccinate lyase (ASL) Lyase 1 family P04424
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Tripartite motif-containing protein 3 (TRIM3) TRIM/RBCC family O75382

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
2-(N-morpholino)Ethanesulfonic Acid Small molecular drug DB03814
Arginine Small molecular drug DB00125
Argininosuccinate . DB02267

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402