Details of the Target

General Information of Target

Target ID LDTP02003
Target Name DNA nucleotidylexotransferase (DNTT)
Gene Name DNTT
Gene ID 1791
Synonyms
TDT; DNA nucleotidylexotransferase; EC 2.7.7.31; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKG
FRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMT
GKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCE
FRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAV
LNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLV
SCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQL
LQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSS
WQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKT
KRIFLKAESEEEIFAHLGLDYIEPWERNA
Target Bioclass
Enzyme
Family
DNA polymerase type-X family
Subcellular location
Nucleus
Function
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Uniprot ID
P04053
Ensemble ID
ENST00000371174.5
HGNC ID
HGNC:2983
ChEMBL ID
CHEMBL4810

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.D356N .
COLO792 SNV: p.G114E .
HT115 SNV: p.E264D .
IGR1 SNV: p.K281Ter .
MOLM13 SNV: p.R287Q .
RH41 SNV: p.T169A .
SBC5 Substitution: p.D83N .
SF295 SNV: p.Q422H .
SKMEL5 SNV: p.R245Q .
TOV21G SNV: p.G270D .
WM115 SNV: p.E381K .
WM2664 SNV: p.E381K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y462(20.00)  LDD0260  [1]
DBIA
 Probe Info  		C302(2.00); C403(3.02); C216(1.24); C437(1.40)  LDD3339  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C302(0.00); C216(0.00); C188(0.00); C155(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		C302(0.00); C403(0.00); C216(0.00); C155(0.00)  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		C302(0.00); C216(0.00); C155(0.00); C437(0.00)  LDD0037  [3]
Compound 10
 Probe Info  		C155(0.00); C216(0.00); C437(0.00)  LDD2216  [4]
Compound 11
 Probe Info  		C155(0.00); C216(0.00); C437(0.00)  LDD2213  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 697 C302(2.04); C403(1.84); C216(2.47); C188(1.99)  LDD2245  [2]
 LDCM0023  KB03 697 C302(1.74); C403(3.87); C216(2.94); C188(2.35)  LDD2662  [2]
 LDCM0024  KB05 NALM-6 C302(2.00); C403(3.02); C216(1.24); C437(1.40)  LDD3339  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proto-oncogene c-Rel (REL) . Q04864
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Meiosis-specific protein MEI4 (MEI4) MEI4L family A8MW99

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
2'3'-dideoxyadenosine Triphosphate Small molecular drug DB02189

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279