Details of the Target

General Information of Target

Target ID LDTP01764
Target Name All-trans-retinol dehydrogenase [NAD(+)] ADH1B (ADH1B)
Gene Name ADH1B
Gene ID 125
Synonyms
ADH2; All-trans-retinol dehydrogenase [NAD(+)] ADH1B; EC 1.1.1.105; Alcohol dehydrogenase 1B; Alcohol dehydrogenase subunit beta
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICHTDDHVVSGNLVT
PLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNP
RGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTG
YGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATE
CINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQ
NLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGF
DLLHSGKSIRTVLTF
Target Bioclass
Enzyme
Family
Zinc-containing alcohol dehydrogenase family
Subcellular location
Cytoplasm
Function
Catalyzes the NAD-dependent oxidation of all-trans-retinol and its derivatives such as all-trans-4-hydroxyretinol and may participate in retinoid metabolism. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and its derivatives such as all-trans-4-oxoretinal. Catalyzes in the oxidative direction with higher efficiency. Has the same affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal.
Uniprot ID
P00325
Ensemble ID
ENST00000305046.13
HGNC ID
HGNC:250
ChEMBL ID
CHEMBL3284

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IPC298 SNV: p.G237D .
MFE319 SNV: p.A4T .
MOLT4 SNV: p.T179A .
NCIH446 Insertion: p.I161NfsTer2 .
OVK18 SNV: p.N119D .
RKO SNV: p.S279P .
RL952 SNV: p.T371S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C241(1.11)  LDD3323  [1]
BTD
 Probe Info  		C98(0.41)  LDD2141  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 HuH-1 C241(0.85)  LDD2374  [1]
 LDCM0023  KB03 CFPAC-1 C72(1.34)  LDD2717  [1]
 LDCM0024  KB05 SKMEL24 C241(1.11)  LDD3323  [1]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C98(0.41)  LDD2141  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761