Details of the Target

General Information of Target

Target ID LDTP01679
Target Name Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3 (NDST3)
Gene Name NDST3
Gene ID 9348
Synonyms
HSST3; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3; Glucosaminyl N-deacetylase/N-sulfotransferase 3; NDST-3; hNDST-3; N-heparan sulfate sulfotransferase 3; N-HSST 3) [Includes: Heparan sulfate N-deacetylase NDST3; EC 3.-.-.-; [heparan sulfate]-glucosamine N-sulfotransferase NDST3; EC 2.8.2.8)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSFIMKLHRHFQRTVILLATFCMVSIIISAYYLYSGYKQENELSETASEVDCGDLQHLPY
QLMEVKAMKLFDASRTDPTVLVFVESQYSSLGQDIIMILESSRFQYHIEIAPGKGDLPVL
IDKMKGKYILIIYENILKYINMDSWNRSLLDKYCVEYGVGVIGFHKTSEKSVQSFQLKGF
PFSIYGNLAVKDCCINPHSPLIRVTKSSKLEKGSLPGTDWTVFQINHSAYQPVIFAKVKT
PENLSPSISKGAFYATIIHDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLS
LDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQNLLRAQITNFTFNLGFSGKFYHTGTEE
EDEGDDCLLGSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKKFALEHGIPTDMGYAV
APHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPHLKPARYRRGFIHKNIMVLPRQTCGLF
THTIFYKEYPGGPKELDKSIQGGELFFTVVLNPISIFMTHLSNYGNDRLGLYTFVNLANF
VKSWTNLRLQTLPPVQLAHKYFELFPDQKDPLWQNPCDDKRHRDIWSKEKTCDRLPKFLV
IGPQKTGTTALYLFLVMHPSILSNSPSPKTFEEVQFFNRNNYHRGIDWYMDFFPVPSNVT
TDFLFEKSANYFHSEEAPKRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALKFS
FYEVISAGPRAPSELRALQKRCLVPGWYASHIERWLVYFPPFQLLIIDGQQLRTDPATVM
DEVQKFLGVLPHYNYSEALTFDSHKGFWCQLLEEGKTKCLGKSKGRKYPPMDSDSRTFLS
SYYRDHNVELSKLLHKLGQPLPSWLRQELQKVR
Target Bioclass
Enzyme
Family
Sulfotransferase 1 family, NDST subfamily
Subcellular location
Golgi apparatus membrane
Function
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.
Uniprot ID
O95803
Ensemble ID
ENST00000296499.6
HGNC ID
HGNC:7682

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.L743F .
AN3CA SNV: p.E49K .
C32 SNV: p.H227Y .
CASKI SNV: p.W376Ter .
COLO792 SNV: p.D651N .
HEC1 SNV: p.R461Q .
HT115 SNV: p.S207Y .
Ishikawa (Heraklio) 02 ER SNV: p.I98V .
L428 SNV: p.F182Y .
LN18 SNV: p.L869M .
LOXIMVI SNV: p.E361G .
LS123 SNV: p.M97I .
LS180 SNV: p.L299F .
MOLM16 SNV: p.V84L .
NCIH661 SNV: p.A419S .
OZ SNV: p.G294E .
RL952 SNV: p.Y88C .
SKMEL24 SNV: p.P200L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C577(2.48)  LDD2157  [1]

References

1 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151