Details of the Target

General Information of Target

Target ID LDTP01385
Target Name Nebulette (NEBL)
Gene Name NEBL
Gene ID 10529
Synonyms
C10orf113; LNEBL; Nebulette; Actin-binding Z-disk protein
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRVPVFEDIKDETEEEKIGEEENEEDQVFYKPVIEDLSMELARKCTELISDIRYKEEFKK
SKDKCTFVTDSPMLNHVKNIGAFISEAKYKGTIKADLSNSLYKRMPATIDSVFAGEVTQL
QSEVAYKQKHDAAKGFSDYAHMKEPPEVKHAMEVNKHQSNISYRKDVQDTHTYSAELDRP
DIKMATQISKIISNAEYKKGQGIMNKEPAVIGRPDFEHAVEASKLSSQIKYKEKFDNEMK
DKKHHYNPLESASFRQNQLAATLASNVKYKKDIQNMHDPVSDLPNLLFLDHVLKASKMLS
GREYKKLFEENKGMYHFDADAVEHLHHKGNAVLQSQVKYKEEYEKNKGKPMLEFVETPSY
QASKEAQKMQSEKVYKEDFEKEIKGRSSLDLDKTPEFLHVKYITNLLREKEYKKDLENEI
KGKGMELNSEVLDIQRAKRASEMASEKEYKKDLESIIKGKGMQAGTDTLEMQHAKKAAEI
ASEKDYKRDLETEIKGKGMQVSTDTLDVQRAKKASEMASQKQYKKDLENEIKGKGMQVSM
DIPDILRAKRTSEIYSQRKYKDEAEKMLSNYSTIADTPEIQRIKTTQQNISAVFYKKEVG
AGTAVKDSPEIERVKKNQQNISSVKYKEEIKHATAISDPPELKRVKENQKNISNLQYKEQ
NYKATPVSMTPEIERVRRNQEQLSAVKYKGELQRGTAISDPPELKRAKENQKNISNVYYR
GQLGRATTLSVTPEMERVKKNQENISSVKYTQDHKQMKGRPSLILDTPAMRHVKEAQNHI
SMVKYHEDFEKTKGRGFTPVVDDPVTERVRKNTQVVSDAAYKGVHPHIVEMDRRPGIIVD
LKVWRTDPGSIFDLDPLEDNIQSRSLHMLSEKASHYRRHWSRSHSSSTFGTGLGDDRSEI
SEIYPSFSCCSEVTRPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSMRSMQHSPNLRTYR
AMYDYSAQDEDEVSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYIEFVN
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Binds to actin and plays an important role in the assembly of the Z-disk. May functionally link sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres. Isoform 2 might play a role in the assembly of focal adhesion.
Uniprot ID
O76041
Ensemble ID
ENST00000377122.9
HGNC ID
HGNC:16932

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.Q158Ter DBIA    Probe Info 
AN3CA SNV: p.G892S DBIA    Probe Info 
CAOV3 SNV: p.T404I DBIA    Probe Info 
FTC133 SNV: p.E7Ter; p.L692P DBIA    Probe Info 
G361 SNV: p.E493K DBIA    Probe Info 
HCT116 SNV: p.Y928H .
JURKAT SNV: p.S61P; p.Y688H .
MEC1 SNV: p.L704V DBIA    Probe Info 
MFE319 SNV: p.I714T DBIA    Probe Info 
NALM6 Deletion: p.L289Ter DBIA    Probe Info 
NCIH358 SNV: p.Q934L DBIA    Probe Info 
NUGC3 SNV: p.G978S DBIA    Probe Info 
SAOS2 SNV: p.L177P DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y963(14.13); Y993(9.14)  LDD3495  [1]
DBIA
 Probe Info  		C53(1.11)  LDD3312  [2]
W1
 Probe Info  		N.A.  LDD0236  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 22RV1 C53(1.24)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C53(1.20)  LDD2660  [2]
 LDCM0024  KB05 HMCB C53(1.11)  LDD3312  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamine--tRNA ligase (QARS1) Class-I aminoacyl-tRNA synthetase family P47897
Presenilin-2 (PSEN2) Peptidase A22A family P49810
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
mRNA export factor GLE1 (GLE1) GLE1 family Q53GS7
Huntingtin (HTT) Huntingtin family P42858
Peroxisome assembly protein 26 (PEX26) Peroxin-26 family Q7Z412
Other
Click To Hide/Show 20 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Protein FAM9B (FAM9B) FAM9 family Q8IZU0
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Golgin subfamily A member 6A (GOLGA6A) GOLGA6 family Q9NYA3
Homer protein homolog 3 (HOMER3) Homer family Q9NSC5
Glial fibrillary acidic protein (GFAP) Intermediate filament family P14136
Neurofilament light polypeptide (NEFL) Intermediate filament family P07196
Prelamin-A/C (LMNA) Intermediate filament family P02545
Leucine zipper putative tumor suppressor 2 (LZTS2) LZTS2 family Q9BRK4
Mitotic spindle assembly checkpoint protein MAD1 (MAD1L1) MAD1 family Q9Y6D9
NUT family member 1 (NUTM1) NUT family Q86Y26
Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3) PI3K p85 subunit family Q92569
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) . Q15027
Centrosomal protein of 70 kDa (CEP70) . Q8NHQ1
Coiled-coil domain-containing protein 125 (CCDC125) . Q86Z20
Merlin (NF2) . P35240
Mirror-image polydactyly gene 1 protein (MIPOL1) . Q8TD10
Sperm-associated antigen 5 (SPAG5) . Q96R06
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699
TNF receptor-associated factor 1 (TRAF1) . Q13077

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.