Details of the Target

General Information of Target

Target ID LDTP01121
Target Name Slit homolog 3 protein (SLIT3)
Gene Name SLIT3
Gene ID 6586
Synonyms
KIAA0814; MEGF5; SLIL2; Slit homolog 3 protein; Slit-3; Multiple epidermal growth factor-like domains protein 5; Multiple EGF-like domains protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIP
RNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNK
LQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRAL
RDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFT
LCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLME
IPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSL
VLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISK
GLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKS
KKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLN
DNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRV
FRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINL
LSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQ
LSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLI
DLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPE
GSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPT
HRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCI
QNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVC
ICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVA
HKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQE
PTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGD
NDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPK
SLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKA
LPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHG
KCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSG
EHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQ
CTDGSSFVEEVERHLECGCLACS
Target Bioclass
Transporter and channel
Subcellular location
Secreted
Function May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
Uniprot ID
O75094
Ensemble ID
ENST00000332966.8
HGNC ID
HGNC:11087

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A431 SNV: p.R1177Q .
COLO678 SNV: p.C1519W .
COLO792 SNV: p.W1359Ter .
DU145 SNV: p.K85N; p.E770Ter .
GAMG SNV: p.A21V .
HCT116 SNV: p.L828I .
HCT15 SNV: p.T39A; p.E1429D .
HEC1B SNV: p.K1198N .
ICC12 Substitution: p.R231E .
Ishikawa (Heraklio) 02 ER SNV: p.K296N .
MCAS SNV: p.D753Y .
MCC13 SNV: p.A1394V .
MDST8 SNV: p.S582N .
MIAPACA2 Substitution: p.S380P .
NALM6 SNV: p.A179V .
NCIH1993 SNV: p.P133Q .
NCIH2291 SNV: p.C221S .
NCIH358 SNV: p.T157N .
OVK18 SNV: p.G58D .
REH SNV: p.Q400Ter .
RL SNV: p.K340M .
SBC5 SNV: p.M299I DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C738(5.56)  LDD0080  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
YN-1
 Probe Info  		N.A.  LDD0447  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0281  AC21 HEK-293T C943(0.88)  LDD1520  [5]
 LDCM0289  AC29 HEK-293T C943(1.14)  LDD1528  [5]
 LDCM0298  AC37 HEK-293T C943(1.25)  LDD1537  [5]
 LDCM0307  AC45 HEK-293T C943(1.16)  LDD1546  [5]
 LDCM0312  AC5 HEK-293T C943(0.92)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C943(1.11)  LDD1555  [5]
 LDCM0325  AC61 HEK-293T C943(0.98)  LDD1564  [5]
 LDCM0248  AKOS034007472 HEK-293T C943(1.00)  LDD1511  [5]
 LDCM0409  CL21 HEK-293T C943(1.20)  LDD1613  [5]
 LDCM0422  CL33 HEK-293T C943(1.33)  LDD1626  [5]
 LDCM0435  CL45 HEK-293T C943(1.06)  LDD1639  [5]
 LDCM0448  CL57 HEK-293T C943(0.96)  LDD1651  [5]
 LDCM0461  CL69 HEK-293T C943(0.96)  LDD1664  [5]
 LDCM0475  CL81 HEK-293T C943(1.01)  LDD1678  [5]
 LDCM0484  CL9 HEK-293T C943(1.31)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C943(1.09)  LDD1691  [5]
 LDCM0022  KB02 HCT 116 C738(5.56)  LDD0080  [1]
 LDCM0023  KB03 HCT 116 C738(2.07)  LDD0081  [1]
 LDCM0024  KB05 HCT 116 C738(3.49)  LDD0082  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402