Details of the Target

General Information of Target

Target ID LDTP00974
Target Name Cell adhesion molecule DSCAM (DSCAM)
Gene Name DSCAM
Gene ID 1826
Synonyms
Cell adhesion molecule DSCAM; CHD2; Down syndrome cell adhesion molecule
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MWILALSLFQSFANVFSEDLHSSLYFVNASLQEVVFASTTGTLVPCPAAGIPPVTLRWYL
ATGEEIYDVPGIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKA
VLREPYTVRVEDQKTMRGNVAVFKCIIPSSVEAYITVVSWEKDTVSLVSGSRFLITSTGA
LYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVSDPANSAPSILDGFDHRKAMAGQ
RVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLIENIRPSDSGSYVCEVSNRYG
TAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGTEDQELSWYRNGEILNPGKNV
RITGINHENLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSP
AEPVSLMCNVKGTPLPTITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGG
VYRCTANNSAGVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKN
SNLLPFNHRQVAFENNGTLKLSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQ
PFEFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLS
LMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPT
IVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSM
YLTVKIPAMITSYPNTTLATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTK
EVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKART
ITLRWTMGFDGNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRM
YAKNRIGKSEPSNELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGY
QIGYREYSTGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEII
TTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEI
KNITTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGPPAGVKAAAA
SASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVW
VVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWM
KDSNGTPSLVTIDGRRSIFSNGSFIIRTVKAEDSGYYSCIANNNWGSDEIILNLQVQVPP
DQPRLTVSKTTSSSITLSWLPGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLEN
LKCGTWYKFTLTAQNGVGPGRISEIIEAKTLGKEPQFSKEQELFASINTTRVRLNLIGWN
DGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQEATWYELQMRVCNSAGCAEKQ
ANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLKMLVTISCILVGVLLLFVLLLVVRRRR
REQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLLIEERDTMETIDD
RSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGP
TARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESDSYSVSPSQDTDRARSS
MVSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTS
STPSESGICRFTASPPKPQDGGRVMNMAVPKAHRPGDLIHLPPYLRMDFLLNRGGPGTSR
DLSLGQACLEPQKSRTLKRPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQREGAELG
QAAKMSSSQESLLDSRGHLKGNNPYAKSYTLV
Target Bioclass
Immunoglobulin
Subcellular location
Secreted; Cell membrane
Function
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. Might also collaborate with UNC5C in NTN1-mediated axon repulsion independently of DCC. In spinal cord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. Adhesion molecule that promotes lamina-specific synaptic connections in the retina: expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions.
Uniprot ID
O60469
Ensemble ID
ENST00000400454.6
HGNC ID
HGNC:3039

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
769P SNV: p.A1873S .
A2780 SNV: p.Q1122R .
AU565 SNV: p.R498Ter .
C4II SNV: p.I891T .
COLO792 SNV: p.G1015E .
DANG SNV: p.R196H .
DU145 SNV: p.R1620W .
FADU SNV: p.G1031A .
HCT116 SNV: p.G1458D .
HCT15 SNV: p.E567Ter; p.I977N; p.G1311V .
HEC1 SNV: p.T1146I; p.R1514M .
HEC1B SNV: p.G762C .
HELA SNV: p.S779F .
HKGZCC SNV: p.S1432C .
HT115 SNV: p.S931F .
IGR37 SNV: p.G2001R .
IGR39 SNV: p.G2001R .
Ishikawa (Heraklio) 02 ER Deletion: p.E1093KfsTer6
SNV: p.R196H;p.A421V		 .
JHH4 SNV: p.G701R .
JURKAT SNV: p.G1268R .
K562 SNV: p.L1814M .
KU1919 SNV: p.T1083S .
KYSE150 SNV: p.P596Q .
LOXIMVI SNV: p.D633N .
LS123 SNV: p.R1733K .
LS180 Insertion: p.E1093RfsTer47 .
MCC13 SNV: p.E1439K .
MDAMB157 SNV: p.L1546Q .
MELHO SNV: p.D1302N; p.R1624K; p.T1765N .
MEWO SNV: p.R270K; p.K805Ter .
NCIH1703 SNV: p.Y961Ter .
NCIH2286 SNV: p.E1671Q .
RKO SNV: p.Y59C .
RL952 SNV: p.E64K .
RPMI7951 SNV: p.P717L; p.A1453T .
SKMEL2 SNV: p.A515E; p.A788V .
SKMEL30 SNV: p.Y1246C .
SKOV3 SNV: p.P47S .
SNU1079 SNV: p.N282K .
SW403 SNV: p.L24I .
TCCSUP SNV: p.W1424Ter .
TOV21G Deletion: p.K1877NfsTer8 .
UACC257 SNV: p.G339E .
UACC62 SNV: p.Y1533Ter .
WM793 SNV: p.T1111I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C246(1.14)  LDD2090  [1]
NAIA_4
 Probe Info  		N.A.  LDD2226  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C246(1.14)  LDD2090  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C246(1.06)  LDD2140  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264