Details of the Target

General Information of Target

Target ID LDTP00949
Target Name Phospholipid-transporting ATPase VA (ATP10A)
Gene Name ATP10A
Gene ID 57194
Synonyms
ATP10C; ATPVA; ATPVC; KIAA0566; Phospholipid-transporting ATPase VA; EC 7.6.2.1; ATPase class V type 10A; Aminophospholipid translocase VA; P4-ATPase flippase complex alpha subunit ATP10A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEREPAGTEEPGPPGRRRRREGRTRTVRSNLLPPPGAEDPAAGAAKGERRRRRGCAQHLA
DNRLKTTKYTLLSFLPKNLFEQFHRPANVYFVFIALLNFVPAVNAFQPGLALAPVLFILA
ITAFRDLWEDYSRHRSDHKINHLGCLVFSREEKKYVNRFWKEIHVGDFVRLRCNEIFPAD
ILLLSSSDPDGLCHIETANLDGETNLKRRQVVRGFSELVSEFNPLTFTSVIECEKPNNDL
SRFRGCIIHDNGKKAGLYKENLLLRGCTLRNTDAVVGIVIYAGHETKALLNNSGPRYKRS
KLERQMNCDVLWCVLLLVCMSLFSAVGHGLWIWRYQEKKSLFYVPKSDGSSLSPVTAAVY
SFLTMIIVLQVLIPISLYVSIEIVKACQVYFINQDMQLYDEETDSQLQCRALNITEDLGQ
IQYIFSDKTGTLTENKMVFRRCTVSGVEYSHDANAQRLARYQEADSEEEEVVPRGGSVSQ
RGSIGSHQSVRVVHRTQSTKSHRRTGSRAEAKRASMLSKHTAFSSPMEKDITPDPKLLEK
VSECDKSLAVARHQEHLLAHLSPELSDVFDFFIALTICNTVVVTSPDQPRTKVRVRFELK
SPVKTIEDFLRRFTPSCLTSGCSSIGSLAANKSSHKLGSSFPSTPSSDGMLLRLEERLGQ
PTSAIASNGYSSQADNWASELAQEQESERELRYEAESPDEAALVYAARAYNCVLVERLHD
QVSVELPHLGRLTFELLHTLGFDSVRKRMSVVIRHPLTDEINVYTKGADSVVMDLLQPCS
SVDARGRHQKKIRSKTQNYLNVYAAEGLRTLCIAKRVLSKEEYACWLQSHLEAESSLENS
EELLFQSAIRLETNLHLLGATGIEDRLQDGVPETISKLRQAGLQIWVLTGDKQETAVNIA
YACKLLDHDEEVITLNATSQEACAALLDQCLCYVQSRGLQRAPEKTKGKVSMRFSSLCPP
STSTASGRRPSLVIDGRSLAYALEKNLEDKFLFLAKQCRSVLCCRSTPLQKSMVVKLVRS
KLKAMTLAIGDGANDVSMIQVADVGVGISGQEGMQAVMASDFAVPKFRYLERLLILHGHW
CYSRLANMVLYFFYKNTMFVGLLFWFQFFCGFSASTMIDQWYLIFFNLLFSSLPPLVTGV
LDRDVPANVLLTNPQLYKSGQNMEEYRPRTFWFNMADAAFQSLVCFSIPYLAYYDSNVDL
FTWGTPIVTIALLTFLLHLGIETKTWTWLNWITCGFSVLLFFTVALIYNASCATCYPPSN
PYWTMQALLGDPVFYLTCLMTPVAALLPRLFFRSLQGRVFPTQLQLARQLTRKSPRRCSA
PKETFAQGRLPKDSGTEHSSGRTVKTSVPLSQPSWHTQQPVCSLEASGEPSTVDMSMPVR
EHTLLEGLSAPAPMSSAPGEAVLRSPGGCPEESKVRAASTGRVTPLSSLFSLPTFSLLNW
ISSWSLVSRLGSVLQFSRTEQLADGQAGRGLPVQPHSGRSGLQGPDHRLLIGASSRRSQ
Target Bioclass
Enzyme
Family
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily
Subcellular location
Cell membrane
Function
Catalytic component of P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of phosphatidylcholine (PC) from the outer to the inner leaflet of the plasma membrane. Initiates inward plasma membrane bending and recruitment of Bin/amphiphysin/Rvs (BAR) domain-containing proteins involved in membrane tubulation and cell trafficking. Facilitates ITGB1/beta1 integrin endocytosis, delaying cell adhesion and cell spreading on extracellular matrix. Has low flippase activity toward glucosylceramide (GlcCer).
Uniprot ID
O60312
Ensemble ID
ENST00000356865.11
HGNC ID
HGNC:13542

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.T1205PfsTer4 .
DETROIT562 SNV: p.P224H .
HCT15 SNV: p.W1263Ter; p.G1270C; p.K1414N .
HELA SNV: p.G15E .
HGC27 SNV: p.C55G .
HT SNV: p.L599V .
HT115 SNV: p.R809C; p.R1084Q .
HT1197 SNV: p.P1189R .
HUPT3 SNV: p.R85H .
ICC12 SNV: p.P1261L .
IGROV1 SNV: p.I247V .
Ishikawa (Heraklio) 02 ER Insertion: p.E260RfsTer11
SNV: p.G7V		 .
KARPAS299 SNV: p.G349V .
KMCH1 SNV: p.P642Q DBIA    Probe Info 
KMRC3 Deletion: p.W333Ter .
LS180 SNV: p.K747E; p.K1016R .
LUDLU1 SNV: p.D1031E .
MCC13 SNV: p.R1479Q DBIA    Probe Info 
MCC26 SNV: p.L994F DBIA    Probe Info 
MEWO SNV: p.S646F; p.P1353S .
MFE319 SNV: p.Q884Ter; p.F993I .
NCIH2172 Deletion: p.R296AfsTer11 .
NCIH446 Deletion: p.K254RfsTer11 .
NUGC3 SNV: p.K996Ter .
ONS76 SNV: p.C932Y .
REH SNV: p.A1211T .
SNGM SNV: p.G1045S .
SUPT1 SNV: p.V493A .
SW1783 SNV: p.R457H .
SW480 SNV: p.T1116A .
TF1 SNV: p.E303G .
TOV21G Substitution: p.L96P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C1318(5.36)  LDD2157  [1]
DBIA
 Probe Info  		C246(1.63)  LDD3382  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 ABC-1 C246(1.34)  LDD2262  [2]
 LDCM0023  KB03 ABC-1 C246(1.38)  LDD2679  [2]
 LDCM0024  KB05 OVCAR-4 C246(1.63)  LDD3382  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell cycle control protein 50A (TMEM30A) CDC50/LEM3 family Q9NV96

References

1 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840