Details of the Target

General Information of Target

Target ID LDTP00920
Target Name SH3 domain-binding protein 5 (SH3BP5)
Gene Name SH3BP5
Gene ID 9467
Synonyms
SAB; SH3 domain-binding protein 5; SH3BP-5; SH3 domain-binding protein that preferentially associates with BTK
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDAALKRSRSEEPAEILPPARDEEEEEEEGMEQGLEEEEEVDPRIQGELEKLNQSTDDIN
RRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQ
DFQRATEVLRAAKETISLAEQRLLEDDKRQFDSAWQEMLNHATQRVMEAEQTKTRSELVH
KETAARYNAAMGRMRQLEKKLKRAINKSKPYFELKAKYYVQLEQLKKTVDDLQAKLTLAK
GEYKMALKNLEMISDEIHERRRSSAMGPRGCGVGAEGSSTSVEDLPGSKPEPDAISVASE
AFEDDSCSNFVSEDDSETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLDLPSPVSLSEFGM
MFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKANNNRGLSSSSGSGGSSKSQSST
SPEGQALENRMKQLSLQCSKGRDGIIADIKMVQIG
Target Bioclass
Other
Family
SH3BP5 family
Subcellular location
Cytoplasmic vesicle membrane
Function
Functions as a guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25. Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
Uniprot ID
O60239
Ensemble ID
ENST00000383791.8
HGNC ID
HGNC:10827

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.P363S; p.L404I .
IM95 SNV: p.S308T .
Ishikawa (Heraklio) 02 ER SNV: p.M266T .
SW1116 SNV: p.K202T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C438(3.30)  LDD3332  [1]
5E-2FA
 Probe Info  		N.A.  LDD2235  [2]
CY-1
 Probe Info  		K240(0.00); K244(0.00)  LDD0246  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 KATO III C438(1.91)  LDD2416  [1]
 LDCM0023  KB03 KATO III C438(1.96)  LDD2833  [1]
 LDCM0024  KB05 MKN45 C438(3.30)  LDD3332  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tyrosine-protein kinase BTK (BTK) Tyr protein kinase family Q06187
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleosome assembly protein 1-like 2 (NAP1L2) Nucleosome assembly protein (NAP) family Q9ULW6

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.