Details of the Target

General Information of Target

Target ID LDTP00454
Target Name Phytanoyl-CoA dioxygenase, peroxisomal (PHYH)
Gene Name PHYH
Gene ID 5264
Synonyms
PAHX; Phytanoyl-CoA dioxygenase, peroxisomal; EC 1.14.11.18; Phytanic acid oxidase; Phytanoyl-CoA alpha-hydroxylase; PhyH
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEQLRAAARLQIVLGHLGRPSAGAVVAHPTSGTISSASFHPQQFQYTLDNNVLTLEQRKF
YEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITK
VQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHY
FPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYE
ENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENI
EKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL
Target Bioclass
Enzyme
Family
PhyH family
Subcellular location
Peroxisome
Function
Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs.
Uniprot ID
O14832
Ensemble ID
ENST00000263038.9
HGNC ID
HGNC:8940

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.K59Q .
NALM6 SNV: p.Q122R DBIA    Probe Info 
U2OS SNV: p.V305I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.84  LDD0403  [1]
DBIA
 Probe Info  		C34(2.51)  LDD3310  [2]
ATP probe
 Probe Info  		N.A.  LDD0199  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [4]
STPyne
 Probe Info  		N.A.  LDD0009  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.84  LDD0403  [1]
 LDCM0022  KB02 8305C C34(1.35)  LDD2248  [2]
 LDCM0023  KB03 8305C C34(1.34)  LDD2665  [2]
 LDCM0024  KB05 COLO792 C34(2.51)  LDD3310  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha/beta hydrolase domain-containing protein 17C (ABHD17C) ABHD17 family Q6PCB6
Cell division cycle protein 23 homolog (CDC23) APC8/CDC23 family Q9UJX2
COP9 signalosome complex subunit 3 (COPS3) CSN3 family Q9UNS2
E3 SUMO-protein ligase PIAS1 (PIAS1) PIAS family O75925
RING finger protein 112 (RNF112) GB1/RHD3 GTPase family Q9ULX5
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
LIM/homeobox protein Lhx6 (LHX6) . Q9UPM6
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytokine receptor-like factor 3 (CRLF3) Cytokine receptor-like factor 3 family Q8IUI8
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM9B (FAM9B) FAM9 family Q8IZU0
TNF receptor-associated factor 5 (TRAF5) TNF receptor-associated factor family O00463
Erythroid differentiation-related factor 1 (EDRF1) . Q3B7T1
Kelch-like protein 20 (KLHL20) . Q9Y2M5
Melanoma-associated antigen 11 (MAGEA11) . P43364
Sperm-associated microtubule inner protein 4 (SPMIP4) . Q8N865

The Drug(s) Related To This Target

Approved
Click To Hide/Show 4 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Antihemophilic Factor Human Recombinant BiotechDrug DB00025
Ascorbic Acid Small molecular drug DB00126
Lonoctocog Alfa . DB13998
Moroctocog Alfa . DB13999

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764