Details of the Target

General Information of Target

Target ID LDTP00200
Target Name Eyes absent homolog 2 (EYA2)
Gene Name EYA2
Gene ID 2139
Synonyms
EAB1; Eyes absent homolog 2; EC 3.1.3.48
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVELVISPSLTVNSDCLDKLKFNRADAAVWTLSDRQGITKSAPLRVSQLFSRSCPRVLPR
QPSTAMAAYGQTQYSAGIQQATPYTAYPPPAQAYGIPSYSIKTEDSLNHSPGQSGFLSYG
SSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGS
SYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDR
PHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTT
SVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAP
GANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLRAELEAL
TDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGK
ESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL
Target Bioclass
Enzyme
Family
HAD-like hydrolase superfamily, EYA family
Subcellular location
Cytoplasm
Function
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1.
Uniprot ID
O00167
Ensemble ID
ENST00000327619.10
HGNC ID
HGNC:3520
ChEMBL ID
CHEMBL1293275

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.A415V .
DU145 SNV: p.E536Ter .
GB1 SNV: p.E481K .
HCT15 SNV: p.T298I .
HS936T Substitution: p.V370K .
JURLMK1 SNV: p.S482T .
MFE296 Deletion: p.F467SfsTer3 .
NCIH1048 SNV: p.A510V .
NCIH1792 SNV: p.T141K .
RKO SNV: p.D353G .
SKMEL24 SNV: p.P453S .
SKMEL30 SNV: p.G263E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD2156  [1]
Methacrolein
 Probe Info  		N.A.  LDD0218  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
Probable E3 ubiquitin-protein ligase DTX2 (DTX2) Deltex family Q86UW9
Testis-specific serine/threonine-protein kinase 3 (TSSK3) CAMK Ser/Thr protein kinase family Q96PN8
Cyclin-dependent kinase 18 (CDK18) CMGC Ser/Thr protein kinase family Q07002
Dual specificity protein phosphatase 21 (DUSP21) Protein-tyrosine phosphatase family Q9H596
TNF receptor-associated factor 4 (TRAF4) TNF receptor-associated factor family Q9BUZ4
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) . Q13526
Sulfite oxidase, mitochondrial (SUOX) . P51687
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cation channel sperm-associated protein 1 (CATSPER1) Cation channel sperm-associated (TC 1.A.1.19) family Q8NEC5
Transcription factor
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA-binding protein RFX2 (RFX2) RFX family P48378
Homeobox protein SIX1 (SIX1) SIX/Sine oculis homeobox family Q15475
Paired box protein Pax-9 (PAX9) . P55771
Pogo transposable element with ZNF domain (POGZ) . Q7Z3K3
Other
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Atos homolog protein B (ATOSB) ATOS family Q7L5A3
Mediator of RNA polymerase II transcription subunit 25 (MED25) Mediator complex subunit 25 family Q71SY5
Tektin-4 (TEKT4) Tektin family Q8WW24
Tektin-5 (TEKT5) Tektin family Q96M29
U1 small nuclear ribonucleoprotein C (SNRPC) U1 small nuclear ribonucleoprotein C family P09234
Vacuolar protein sorting-associated protein 37C (VPS37C) VPS37 family A5D8V6
BAG family molecular chaperone regulator 3 (BAG3) . O95817
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
Galectin-4 (LGALS4) . P56470
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
IQ motif and ubiquitin-like domain-containing protein (IQUB) . Q8NA54
Protein TFG (TFG) . Q92734

References

1 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.