Details of the Target

General Information of Target

Target ID LDTP00095
Target Name Sphingosine-1-phosphate transporter MFSD2B (MFSD2B)
Gene Name MFSD2B
Gene ID 388931
Synonyms
Sphingosine-1-phosphate transporter MFSD2B; Major facilitator superfamily domain-containing protein 2B; hMfsd2b
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPPAPAAKGSPQPEPHAPEPGPGSAKRGREDSRAGRLSFCTKVCYGIGGVPNQIASSA
TAFYLQLFLLDIAQIPAAQVSLVLFGGKVSGAAADPVAGFFINRSQRTGSGRLMPWVLGC
TPFIALAYFFLWFLPPFTSLRGLWYTTFYCLFQALATFFQVPYTALTMLLTPCPRERDSA
TAYRMTVEMAGTLMGATVHGLIVSGAHRPHRCEATATPGPVTVSPNAAHLYCIAAAVVVV
TYPVCISLLCLGVKERPDPSAPASGPGLSFLAGLSLTTRHPPYLKLVISFLFISAAVQVE
QSYLVLFCTHASQLHDHVQGLVLTVLVSAVLSTPLWEWVLQRFGKKTSAFGIFAMVPFAI
LLAAVPTAPVAYVVAFVSGVSIAVSLLLPWSMLPDVVDDFQLQHRHGPGLETIFYSSYVF
FTKLSGACALGISTLSLEFSGYKAGVCKQAEEVVVTLKVLIGAVPTCMILAGLCILMVGS
TPKTPSRDASSRLSLRRRTSYSLA
Target Bioclass
Transporter and channel
Family
Major facilitator superfamily
Subcellular location
Cell membrane
Function
Lipid transporter that specifically mediates export of sphingosine-1-phosphate in red blood cells and platelets. Sphingosine-1-phosphate is a signaling sphingolipid and its export from red blood cells into in the plasma is required for red blood cell morphology. Sphingosine-1-phosphate export from platelets is required for platelet aggregation and thrombus formation. Mediates the export of different sphingosine-1-phosphate (S1P) species, including S1P(d18:0) (sphinganine 1-phosphate), S1P (d18:1) (sphing-4-enine 1-phosphate) and S1P (d18:2) (sphinga-4E,14Z-dienine-1-phosphate) (Probable). Release of sphingosine-1-phosphate is facilitated by a proton gradient. In contrast, cations, such as sodium, are not required to drive sphingosine-1-phosphate transport (Probable). In addition to export, also able to mediate S1P import. Does not transport lysophosphatidylcholine (LPC) (Probable).
Uniprot ID
A6NFX1
Ensemble ID
ENST00000338315.6
HGNC ID
HGNC:37207

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C42(2.48)  LDD2099  [1]
Acrolein
 Probe Info  		N.A.  LDD0217  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C42(2.48)  LDD2099  [1]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C42(2.75)  LDD2107  [1]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C42(1.24)  LDD2109  [1]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C42(3.41)  LDD2119  [1]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C42(1.71)  LDD2127  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C42(2.70)  LDD2136  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C42(2.33)  LDD2140  [1]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C42(4.37)  LDD2144  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.